Cíxidos y dérbidos (Hemiptera) vectores putativos de fitoplasma del grupo 16Sr-IV
Contenido principal del artículo
Los fitoplasmas del grupo 16SrIV causan enfermedades tipo amarillamiento letal (STAL) en palmas a nivel mundial. El fitoplasma del amarillamiento letal del cocotero (ALC) se conoce como ‘Candidatus Phytoplasma palmae’ (16SrIV-A). Al igual que en el ALC, muchas enfermedades causadas por fitoplasma a nivel mundial, sus vectores no han sido identificados. El único vector del fitoplasma del ALC incriminado es Haplaxius crudus (Hemiptera: Cixiidae). El objetivo del presente estudio fue determinar la presencia del fitoplasma 16SrIV en cíxiidos y dérbidos asociados a palmas (Adonidia merrillii y Cocos nucifera) positivas a fitoplasmas. Se realizaron capturas de insectos asociados a estas palmas durante la mañana y tarde. La presencia de fitoplasma fue determinada por PCR en tiempo real, empleando los cebadores TaqMan LY16S-ANYF (GCTAAGTCCCCACCATAACGT) y LY16S-ANYR (CGTGTCGTGAGAT-GTTAGGTTAAGT); sonda LY16S-ANYM (FAMCCCCTGTCGTTAATTG-NFQ). No se detectó la presencia de fitoplasma del grupo 16SrIV en H. crudus aunque sí se mostró su presencia en H. skarphion (Hemiptera: Cixiidae), Oecleus snowi (Hemiptera: Cixiidae) y Persis foveatis (Hemiptera: Derbidae). Estos resultados sugieren que estas tres especies pueden ser potenciales vectores del fitoplasma del grupo 16 SrIV en palmas.
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Aceptado 2020-07-15
Publicado 2020-07-15
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